Enzymatic hydrolysis of UO using lipase is one of the promising approaches to produce free fatty acids and glycerol, both of which are highly utilized especially in the oleochemical industries.
Hydrolysis of coconut oil (VCO) had been carried out by using an immobilised lipase from Mucor miehei (Lipozyme) in a water-jacketed batch reactor. The kinetic of the hydrolysis was investigated by varying the parameters such as VCO concentration, enzyme loading, water content, and reaction temperature.
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The kinetics of enzymatic hydrolysis of rice bran oil in isooctane by immobilized Candida rugosa lipase in a batch reactor showed competitive inhibition by isooctane with a dissociation constant, KI, of 0.92 m. Continuous hydrolysis of rice bran oil was performed in recycling, packed bed reactor with 4352 U of immobilized lipase; the optimum recycle ratio was 9 and the operational half-life
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Semicontinuous hydrolysis of olive oil using immobilized lipase was also accomplished in a packed-bed reactor with a recycling system. In this reactor, immobilized lipase was observed to have the sufficient activity and stability.
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Both oils presented maximum hydrolysis degree around 7% after 4 h of reaction using immobilized lipase. However, for the reactions performed by free lipase, the highest hydrolysis degree was found for olive oil (up to 12%).
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The FFA production reach maximum at 200 rpm indicating that the optimum mixing speed for hydrolysis of J. curcas oil using the immobilized matrix is 200 rpm. Higher mixing rate consign the enzyme to shear stress causing enzyme denaturation [34] Hydrolysis of waste cooking oil was carried out using Candida rugosa lipase.
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Operational stability of immobilized lipase. After each batch reaction (one batch was 60 h), immobilized lipase was filtered, washed with phosphate buffer (2 脳 25 mL, 5 min, at 30 掳C) to remove glycerol in the carrier and dried at room temperature. The dried immobilized lipase was used in the next batch reaction composed of fresh substrates.
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TLL was immobilized on Fe 3 O 4 /Au magnetic nanoparticles, and the resulting immobilized lipase was used to catalyze the production of biodiesel from tomato seed oil. Results demonstrated that the conversion rate of biodiesel reached up to 97.4% using 10% of the immobilized lipase (w/w of oil).
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Knothe et al. reported the biodiesel production from sunflower oil using petroleum ether as solvent with immobilized lipase from Pseudomonas fluorescens, reaching 99% yield when the alcohol used was methanol, ethanol, or 1-butanol. In the absence of solvent, yields were reduced to 3%, 70, and 76%, respectively.
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To improve the reusability and organic solvent tolerance of microbial lipase and expand the application of lipase (hydrolysis, esterification, and transesterification), we immobilized marine microbial lipase using different methods and determined the properties of immobilized lipases. Considering the activity and cost of immobilized lipase, the concentration of lipase was fixed at 2 mg
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Lipase-catalyzed synthesis of biodiesel from black soldier fly (Hermetica illucens): Optimization by using response surface methodology. Y. Fu, P. Lv, X. WangSynthesis of biodiesel from waste cooking oil using immobilized lipase in fixed bed reactor. Energy Convers Manage, 50 (2009), pp. 668-673. Google Scholar
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Lipase from Nigella sativa seeds was immobilized by adsorption on Celite 535 from phosphate buffer solutions varying pH values of 5.0.0 at 25 掳C. Langmuir isotherms described the adsorption equilibria well for lipase adsorption at all pH range. The saturation capacity for adsorption of lipase increased from 14.5 to 24.3 mg g 鈭? Celite as the adsorption pH was reduced from 8 to 5, but
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SUMMARY. In the current investigation the crude oil of Nigella sativa was extracted from seeds using hydraulic and screw pressing techniques. Different parameters were evaluated in order to find out the appropriate technique to enhance the physicochemical and functional-related characteristics of the extracted crude oil.
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Biodiesel fuel production via transesterification of oils using lipase biocatalyst. P. cepacia lipase immobilized on celite was used by Shah & Gupta (2007) the effect of a varying amounts of buffer on the ethanolysis reaction of Jatropha oil using C. viscosum lipase and the buffer salt concentration was optimized.
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Partial purification of nigella sativa L. Seed lipase and its application in hydrolytic reactions. Enrichment of 纬-linolenic acid from borage oil seed as the lipase source.The hydrolysis
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Esters were synthesized via the alcoholysis of red pitaya seed oil with oleyl alcohol catalyzed by immobilized lipase, Lipozyme RM IM. The effects of synthesis parameters, including temperature, time, substrate molar ratio and enzyme loading, on the yield and productivity of esters were assessed using a central com
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Knothe et al. reported the biodiesel production from sunflower oil using petroleum ether as solvent with immobilized lipase from Pseudomonas fluorescens, reaching 99% yield when the alcohol used was methanol, ethanol, or 1-butanol. In the absence of solvent, yields were reduced to 3%, 70, and 76%, respectively.
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Biodiesel fuel production via transesterification of oils using lipase biocatalyst. P. cepacia lipase immobilized on celite was used by Shah & Gupta (2007) the effect of a varying amounts of buffer on the ethanolysis reaction of Jatropha oil using C. viscosum lipase and the buffer salt concentration was optimized.
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Lipase from hazelnut seed more specified versus triolein and tributyrin and olive oil among the nature oils as substrate. The enzyme activity was measured by using 0.1 ml of enzyme solution for 5 min. To determine the storage stability of lipase from hazelnut seed, the activity assays carried out for a period of one year. it was observed that about
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Sacha inchi (Plukenetia volubilis) oil has high polyunsaturated fatty acids content.The hydrolysis of this oil is an efficient way to obtain desirable free fatty acids (FFA). The optimization of parameters was carried out according to the maximum production of FFA using two enzymatic hydrolysis processes.
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Read "Partial purification of nigella sativa L. Seed lipase and its application in hydrolytic reactions. Enrichment of 纬-linolenic acid from borage oil, Journal of the American Oil Chemists' Society" on DeepDyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips.
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Background and Sources: Lipase enzyme is a naturally occurring enzyme found in the stomach and pancreatic juice. Its function is to digest fats and lipids, helping to maintain correct gallbladder function. Lipase is the one such widely used and versatile enzyme.
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According to these researchers, fatty acids can be obtained from the hydrolysis of palm oil by crushed castor beans; the hydrolysis of palm oil by an acetone extract from castor beans; the hydrolysis of pine seed oil by crushed castor beans and the hydrolysis of palm oil by the lipases present in the fruit itself.
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Esters were synthesized via the alcoholysis of red pitaya seed oil with oleyl alcohol catalyzed by immobilized lipase, Lipozyme RM IM. The effects of synthesis parameters, including temperature, time, substrate molar ratio and enzyme loading, on the yield and productivity of esters were assessed using a central com
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Knothe et al. reported the biodiesel production from sunflower oil using petroleum ether as solvent with immobilized lipase from Pseudomonas fluorescens, reaching 99% yield when the alcohol used was methanol, ethanol, or 1-butanol. In the absence of solvent, yields were reduced to 3%, 70, and 76%, respectively.
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FTB Food Technol Biotechnol Food Technology and Biotechnology Food Technol. Biotechnol. 1330-9862 1334-2606 University of Zagreb Faculty of Food Technology and
Get PriceMagnetically immobilized papain is prepared by magnetic Fe3O4/P (GMA-EDGMA-St) composite carrier with the addition of 12.0 mg/mL papain, 5.0% glutaral
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These applications include (i) selective detection of sesamol content in sesame seeds (black seed, #1 and white seed, #2) and oils (#35); (ii) biocatalytic reduction and sensing of H 2 O 2 utilizing the horseradish peroxidase (HRP)-based enzyme system immobilized electrode; and (iii) analysis of electrochemical immunosensing of white spot
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The free and immobilized enzymes were characterized. pH and temperature profiles showed maximum activity at pH 6.6 and 45掳C, for both free and immobilized enzymes. There was a gain in thermal stability with enzyme immobilization and there was an increase of about four times in the half-life of the immobilized derivative at 45掳C (from 0.43 h
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cating lipase production were selected for further studies. Based on the qualitative analysis test, the fungal strains that exhibited the highest yellow zones were fur-ther screened using SSF for quantitative enzyme assay. A mass of 5 g of wheat bran was added into 250-mL Erlen-meyer flasks, containing synthetic oil-based (SOB) medi-
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纬-Linolenic acid (Z,Z,Z-6,9,12-octadecatrienoic acid), a very important polyunsaturated fatty acid is found in the free fatty acid fraction prepared by the hydrolysis of borage oil. Our aim was to enrich this fraction in 纬-linolenic acid using selective esterification. Candida rugosa lipase was used as catalyst after immobilization on the following ion-exchange resins: Amberlite IRC50, IRA35
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